Born in Paris, Matthieu Gauci-Ancelin studied with Sophie Cherrier and Vincent Lucas at Conservatoire de Paris , and at the Karajan Academy of Berlin Philharmonic orchestra with Andreas Blau. He received at that time also precious advices from Emmanuel Pahud who became one of his mentor.

Kirill Petrenko appointed him principal Flute of the Komische Oper Berlin at the age of 21. Matthieu hold later the same position at Swedish Radio symphony Orchestra with Daniel Harding. He also plays regularly as guest principal Flute with Berlin Philharmonic since 2007, as well as with London Symphony Orchestra, Munich Philharmonic, Staatskapelle Dresden, 

Mahler Chamber Orchestra and many others. He is since 2019 member of Saito Kinen Orchestra (Seiji Ozawa).

As a soloist, Matthieu performed with many orchestras including Prague Symphony Orchestra, Deutsches Kammerorchester, Sinfonia Varsovia, orchestra of the Komische Oper, with conductors such as Markus Poschner, Henrik Nanasi, Pietari Inkinen or 

Sir Roger Norrington.

He is also an active chamber musician, and was lucky to play with Elena Bashkirova,

Francois Dumont, Adam Laloum, Nathalia Milstein, Lars Vogt, Anton Barakhovsky, Alexander Sitkovetsky, Lucas Macias Navarro, and the Modigliani Quartett.

He was teacher at Hochschule Hanns Eisler from 2017 to 2019, and has been recently appointed Professor at university of Music and Performing Arts Graz  in Austria.